Plasmid

Part:BBa_K3602020:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


Cas13a, composite part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3059
    Illegal BglII site found at 3167
    Illegal BglII site found at 3654
    Illegal BamHI site found at 325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1744
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 212
    Illegal BsaI.rc site found at 2896


Design Notes

This cas13a part is the one from Gootenberg et al. 2017, which originates from LwC2c2. (https://science.sciencemag.org/content/356/6336/438) The RBS, terminator and promoter is from the iGEM Parts page.

A positively regulated promoter (BBa_R0011) was used since Cas13a can be toxic to a cell. With the promoter protein production can be regulated so the expressing bacteria will not die.


Source

A positively regulated promoter (BBa_R0011) was used since Cas13a can be toxic to a cell. With the promoter protein production can be regulated so the expressing bacteria will not die.

References